RESEARCH: Dynamic Adaptation of the Mitochondrial Proteome upon Stress and Metabolic Switches, Metabolic Signalling, Mitochondria, Signal Transduction, Cell Biology, Nervous System Diseases, Oncogenic Signalling

METHODS: Mitochondrial and Submitochondrial Proteomics and Phosphoproteomics, Global Kinome Profiling for Mitochondrial Signalling Pathways, Import and Assembly of Mitochondrial Proteins, Extracellular Metabolic Flux Analysis

TECHNOLOGIES: Seahorse XFe96 Flux Analyzer (Agilent)

RESEARCH: Cancer Metabolism, Mathematical Modelling, Signal Transduction, Developmental Biology, Immune System, Synthetic Biology

METHODS: Dynamic Pathway Modelling, Parameter Estimation in Differential Equations, Uncertainty Analysis, Experimental Design

TECHNOLOGIES: High Performance Computing

RESEARCH: Immunometabolism (Innate and Adaptive Immunity), Host-Microbiota-Crosstalk via Microbial Metabolites in Inflammatory Diseases, Infectious Diseases and Sepsis, Cancer, Nervous System Diseases, Cardiovascular Diseases, and Kidney Diseases

METHODS: Screening of Bioactive Microbial Molecules (Global Metabolomics, Complex Lipids, SCFA) Outsourced and via Collaborations, Multi-Omics Data Integration (e.g. Metabolomics, CyTOF, Shotgun Metagenomics) In-House and via Collaborations, Animal Models with Natural Microbiota (Wildlings) 

TECHNOLOGIES: BCON BSL-3 BioContainment Rodent Housing System (Plexx), Rodent Surgery Units (Including Microscopic Surgery), Fluorescent-based Cellaca MX High-throughput 24 Sample Automated Cell Counter (Nexcelom Bioscience)

RESEARCH: Infectious Diseases, Mycobacteria, Mycobacterial Metabolite Transporters, Metabolic Signalling, Signal Transduction, Extracellular Nanocompartments, Synthetic Biology, Mycobacterial Membranes, Antibiotics, Drug or Photosensitizer-Metabolite Conjugates and Organometallic Complexes

METHODS: Genetic Manipulation of Metabolism, Small Molecule Screening, Targeted Metabolomics by MS, Protein Isolation and Characterization, Proteomics (in collaboration), Molecular Biology, Microbiology, Organic Synthesis

TECHNOLOGIES: LED array 96-well plate (Teleopt), ChemStudio (Analytics Jena)

RESEARCH: Biochemistry, Respiration, Energy Conversion, Enzyme Complexes of Respiration: Structure/Function, Biogenesis and Inhibitors, (Ubi)Quinone Chemistry, Reactive Oxygen Species, Structural Biology, Iron-Sulphur clusters, Mitochondria, Bacteria

METHODS: Genetic Manipulation of Microorganisms, Purification of Membrane Proteins, Protein Stability, Inhibitor screening, Protein labeling (EPR-, fluorescent- and IR-probes), Liposome techniques, He-temp. EPR-spectroscopy, Cryo-EM (via collaboration)

TECHNOLOGIES: 10L Fermenter (Bioengineering), Äkta Protein Purification Systems (GE Healthcare), He-temp. X-Band EPR (Bruker), Fluorescence DSC (PerkinElmer), Fluorescence Imaging (Vilber), Oxygen Electrode (HansaTech), Protein Electrochemistry (ProSense)

RESEARCH: Signal Transduction, Metabolic Signalling, Mitochondria, Cell Biology, Angiogenesis, Retinal Vasoproliferative Disease

METHODS: Pharmacologic Manipulation of Metabolism, Extracellular Metabolic Flux Analysis (via collaboration), mouse models of retinal vasoproliferative disease (mouse model of oxygen-induced retinopathy, VLDLR Knockout mouse model, Laser-CNV model), RNAseq analysis (outsourced), untargeted Metabolomics (via collaboration)

RESEARCH: Enzymology of Tetrapyrrole Biosynthesis, Anaerobic Energy Metabolism in Prokaryotes, Posttranslational Protein Modification, Protein Biochemistry, Radical Enzymes, Iron-Sulfur Cluster Proteins

METHODS: Aerobic and Anaerobic Protein Production and Purification, HPLC Analysis of Small Molecules, Multi-Enzyme Synthesis, UV/Visible Absorption Spectroscopy, Microscale Thermophoresis, Molecular Biology, EPR and Mössbauer Spectroscopy (via collaboration)

TECHNOLOGIES: Monolith NT.Automated (Nanotemper)

RESEARCH: Mathematical Modelling, Metabolic Signaling Networks, Link to Microbiome, Link to Proteome, Methods for Single Cell Analyses

METHODS: Mathematical Models, Benchmarking and Optimal Selection of Computational Methods, Development of Novel Analysis Approaches

TECHNOLOGIES: High Performance Computing

RESEARCH: Glucose Metabolism, Endocrine Pancreas Regeneration and Signal Transduction, Stem Cell Biology, Adipose Tissue Biology, Type 1 Diabetes, Type 2 Diabetes, Gestational Diabetes, Obesity, Incretin Hormones, Clinical Trials in Diabetes and Metabolism, Novel Diabetes Medications, Insulin Research, Cardiovascular Disease, Inflammation, NAFLD

METHODS: Cell Biology, Animal Models for Obesity and Diabetes, Pharmacologic and Genetic Manipulation of Metabolism, Clinical Trials in Patients, Endoscopic bariatric interventions

TECHNOLOGIES: Clinical Trial Unit, Phase I-IV, Glucose Clamps in Animals and Humans

RESEARCH: Metabolite signaling, Metabolite transport, Structure and function of Proteins, Channels, Transporters, Receptors, (Metallo-) Enzymes, Transport mechanisms, Receptor activation, Signal transduction, Protein-protein interaction

METHODS: Protein engineering and isolation, Affinity and size-exclusion chromatography, Molecular Biology, X-ray crystallography, cryo-EM, Proteoliposomes, Lipids, Detergents, Nanoparticles, SSM-/PLB-Electrophysiology, Microscale thermophoresis, Stopped-flow kinetics

TECHNOLOGIES: Planar Lipid Bilayer Electrophysiology (Warner Instr.), Solid-Supported Membrane Electrophysiology (Nanion Tech.), SX-20 Stopped Flow Spectrometer (Applied Photophysics)

RESEARCH: Proteomics, Extra-cellular matrix, Post-translational modifications

METHODS: Liquid chromatography – tandem mass spectrometry

TECHNOLOGIES: Hybrid quadrupole – Orbitrap mass spectrometer, Hybrid linear ion trap – Orbitrap mass spectrometer

RESEARCH: Phosphate Homeostasis, Stringent Response, Phosphorylated Metabolites, Metabolic Messengers, Polyphosphate, Magic Spot Nucleotides, Dinucleoside Polyphosphates, Inositol phosphates and pyrophosphates, Antibiotics

METHODS: Chemical Synthesis, Capillary Electrophoresis and HPLC Mass Spectrometry, Stable Isotope Labeling, Nuclear Magnetic Resonance, Cell Culture

TECHNOLOGIES: CE-MS (Agilent) QQQ, CE-MS (Agilent) QTof, Bruker Avance 400 MHz with cryoprobe

RESEARCH: Cancer Metabolism, Immunometabolism, Acute Leukemia, Allogeneic Stem Cell Transplantation, Graft-versus-host disease, Immunotherapy, Immune checkpoint inhibitor-induced autoimmunity

METHODS: Untargeted Metabolomics and Lipidomics by MS, Pharmacologic and Genetic Manipulation of Metabolism, Extracellular Metabolic Flux Analysis, Acute Leukemia and Allogeneic Stem Cell transplantation mouse models

TECHNOLOGIES: Proficiency in the analysis of raw data from LC-MS metabolomics and lipidomics (targeted, untargeted and tracing experiments)

RESEARCH: Epigenetic regulation of fatty acids synthesis enzymes, Cancer Metabolism, Regulation of lipids uptake, Hypoxia, Mesenchymal transformation in glioblastoma, Epigenetics, Tumor invasion and migration, Regulation of protein cleavage, Tumor angiogenesis.

METHODS: Pharmacologic and Genetic Manipulation of lipid synthesis enzymes, Gene expression analysis by RNAseq and qPCR, Identification of protein-protein interaction, Mice xenografts

TECHNOLOGIES: Seahorse XFe96 Flux Analyzer (Agilent), Hypoxic Workstations i2 and H35 (Whitley)

RESEARCH: Chemical Epigenetics, Immunoncology, Infectious Diseases, Metabolic Signalling, Targeted protein degradation

METHODS: Medicinal Chemistry, Synthesis, Assay development, Small Molecule Screening, Cellular target engagement analysis

TECHNOLOGIES: Organic synthesis infrastructure, Plate washer and readers, 400 MHz NMR for small molecule analysis

RESEARCH: Ribosome-bound Chaperones, Metabolic Signaling, Signal Transduction, Protein Targeting to the ER, Protein Folding

TECHNOLOGIES: Ribosome Profile Analysis, Phos-Tag Gel Analysis, Yeast Genetics

RESEARCH: Wound and Cancer Metabolism, Signal Transduction, Cell Biology, Developmental Biology, Chronic Wounds, Regeneration, Senescence

METHODS: Pharmacologic and Genetic Manipulation of Metabolism, Confocal Microscopy, Metabolome analysis (outsourced)

TECHNOLOGIES: In Vivo Drosophila Models, Intraepithelial and Inter-organ Communication

RESEARCH: Biomimetic Metabolic Pathways, Metabolic Engineering, Multi-enzyme cascades, Biocatalysis, Enzyme Catalysis

METHODS: Genetic Manipulation of Metabolism, Heterologous Expression, Analysis via HPLC and LC-MS

TECHNOLOGIES: HPLC (DAD & ELSD)

RESEARCH: Metabolomics sample preparation, Metabolomics measurements, Automatic data processing

METHODS: Discovery Metabolomics and Lipidomics, Targeted Metabolomics and Lipidomics, 13C label tracing

TECHNOLOGIES: GC-MS (Agilent), LC-QQQ-MS (Agilent), LC-QTOF-MS (Bruker)

RESEARCH: Inborn errors of metabolism, Genetic modifiers of metabolism, Sulfur-metabolism, Redox signalling, Cofactor enzymology and trafficking, Nutrition, Nutritional and Genetic deficiencies of vitamin B12, multiorgan-metabolic integration

METHODS: Metabolic labeling in cell culture, Targeted metabolomic profiling by mass spectrometry, Characterization of pathogenic protein variants, Protein-cofactor interactions, Rapid kinetics by stopped-flow, Biomarker discovery and standardization

TECHNOLOGIES: UHPLC Nexera X2/QTRAP Sciex 6500+ MS, HPLC -TSQ Vantage Triple Quadrupole MS, GC-MS system (7890 GC system coupled to a 5975C mass spectrometer, Agilent), Stopped-Flow with double mixer and dual UV-Vis/Fluorescence detection

RESEARCH: Signaling mechanisms responding to stress and nutrients, ageing and age-related disesases, molecular genetics and biochemistry, C. elegans models of diseases. mTORC1 and mTORC2, SGK-1, signaling mechanisms in tumor biology, programmed cell death, autophagy

METHODS: Genetic dissection of signaling pathways, C. elegans models of human diseases, large scale RNAi screenings, protein interaction studies, DNA and RNA binding, stress biology

TECHNOLOGIES: genome-wide RNAi screenings, mutant screenings, hypoxia chamber, confocal microscopy, C. elegans robotics

RESEARCH: Epigenetic Modification impacting Metabolism, Developmental Processes associated with Metabolic Programs, Nervous System Diseases, Cell Biology, Signal Transduction, Metabolic States driving Stem Cell Specification

METHODS: iPSC, mESC, high throughput data generation and multi-omics analysis, single cell transcriptomics and imaging

TECHNOLOGIES: iPSC and mESC neuronal differentiation; CRISPR/Cas9; RNA- (bulk and single cell), ChIP- and ATAC-seq

RESEARCH: Spatially resolved metabolomics, lipidomics and proteomics, tumor microenvironment, tumor heterogeneity, mass spectrometry and metabolomic data science, mass spectrometry method development

METHODS: MALDI mass spectrometry imaging, computational tools and workflows (in Galaxy platform), multi-omics data integration, machine learning, tissue based mass spectrometry

TECHNOLOGIES: MALDI-TOF/TOF, laser capture microdissection, European Galaxy Server

RESEARCH: Immunometabolism, Metabolic Signalling, Mitochondria, Single-cell Immune Profiling, Mass Cytometry, Imaging Mass Cytometry, Adaptive Immunity, T cells, T cell exhaustion, Signal Transduction, Inflammatory Diseases, Infectious Diseases, Liver Diseases, Cancer Immunology and Immunotherapy

METHODS: Metabolism-directed flow and mass cytometry, imaging Pharmacologic and Genetic Manipulation of Metabolism, Extracellular Metabolic Flux Analysis, Untargeted Metabolomics by MS and NMR (outsourced and via collaborations)

TECHNOLOGIES: Mass cytometry, Imaging Mass cytometry, Hypoxia chambers, Flow cytometry

RESEARCH: Inborn errors of metabolism, mitochondrial fatty acid oxidation disorders, risk and severity profiling, pathophysiology and metabolic switches, organ-specific compensation and environmental modifiers, new therapies

METHODS: Molecular and biochemical phenotyping, functional genomics, targeted metabolomics (disease-specific, pathway-specific, multi-pathway), metabolite panels and metabolic labelling, cell and mouse models, clinical studies,  experimental research

TECHNOLOGIES: MetaboCF (DFG listed, RI_00507); Advanced biochemical diagnostic unit: UHPLC Exion/Triple Quadrupole Sciex 5500+ MS, Acquity UPLC/Waters Quattro Premier Triple Quadrupole MS, HPLC Waters 2695 with DAD, Biochrom 30 and 30+ Amino Acid Analyzers, Agilent GC-MS systems, UV-Vis detectors and plate readers

RESEARCH: Structure and Function of Metabolic Enzymes, , Refactoring of Metabolic Pathways Enzymology, Bioinorganic Chemistry, Structural Biology, Assembly and Biogenesis of Enzymes and Enzyme Complexes

METHODS: Microbiology, Oxic and Anoxic Protein Biochemistry, UV/vis Spectroscopy, EPR Spectroscopy, RT-PCR, Isothermal Titration Calorimetry, Analytical Size-Exclusion Chromatography, Molecular Modelling, Cryo-EM, X-ray Crystallography

TECHNOLOGIES: EPR Sprectrometer (Bruker ElexSys 500), X-ray diffractometer (Rigaku), Cryo-EM (Thermo Fisher)

RESEARCH: Metabolic Imaging, visualization and quantification of adipose tissue and muscle distribution/body composition, link between obesity & cancer, sarcopenia, sarkopenic obesity, obesity paradox, imaging in population-based cohorts and dietary intervention trials

METHODS: Quantification of adipose tissue (visceral & subcutaneous adipose tissue, fat deposits in liver, pancreas, kidney, bone marrow & muscle tissue) with cross-sectional imaging techniques: computed tomography (CT) and magnetic resonance imaging (MRI)

TECHNOLOGIES: CT and MRI scanner with post-processing software for image analysis

RESEARCH: Epigenetic control of metabolic Signalling, Cancer Metabolism, Mitochondria, Immunometabolism, Reprogramming of tumor microenvironment, Immune Sensing, Epigenetics, Structural Biology, Cell Biology, Medicinal Epigenetics and drug development, Cancer

METHODS: Pharmacologic and Genetic Manipulation of Metabolism, Extracellular Metabolic Flux Analysis, Untargeted Metabolomics by MS and NMR (outsourced and via collaborations), 3D structure analyses, structure-based small molecule drug development

TECHNOLOGIES: Seahorse XFe96 Flux Analyzer (Agilent), Hypoxic Workstations i2 and H35 (Whitley), MS and NMR-based metabolomics, crystal structure analyses, medicinal chemistry

RESEARCH: Cancer Metabolism, Metabolic Signalling, Signal transduction, Amino Acids, Developmental Biology, Signal Transduction, Cell Biology, Sarcomas, Oncogenic regulation of tumor metabolism, Antimetabolites as anti-cancer therapeutic agents

METHODS: Pharmacologic and Genetic Manipulation of Metabolism and Signaling in tumor cells, Extracellular Metabolic Flux Analysis, Small Molecule Screening, Untargeted Metabolomics by MS and NMR (outsourced and via collaborations)

TECHNOLOGIES: Mouse and tissue culture models of sarcoma

RESEARCH: Human Genetics, Developmental Biology, Signal Transduction, Cell Biology, Ciliopathies, Kidney Diseases, Skeletal defects, Neurodevelopmental Diseases

METHODS: NGS, Gene editing, Transcriptomics, Proteomics (collaboration with Oliver Schilling. Pathology, University Hospital Freiburg), Metabolomics (collaboration with prof. Spiekerkötter), EM (in external collaboration)

TECHNOLOGIES: CRISPR/Cas gene editing vectors, NGS

RESEARCH: Immunometabolism, Cancer Immunotherapy, Allogeneic Stem Cell Transplantation, Graft-versus-Host Disease, Oncogenic Signaling

METHODS: Pharmacologic and Genetic Manipulation of signaling in tumor cells, animal models for acute leukemia and melanoma, Metabolism, Extracellular Metabolic Flux Analysis, bioluminescence imaging

RESEARCH: Developmental Immunology, Macrophage Development, Macrophage Function, Immunometabolism, Macrophage Metabolism, Metabolic Signalling

METHODS: Ex vivo cell culture platform to study immunometabolism in resident tissue macrophages, tissue normoxic cell cultures, transgenic Drosophila to study conserved immunometabolic signalling

RESEARCH: Immunometabolism, Mitochondria, Microglia, CNS Macrophages, Host-Microbiota-Crosstalk, Nervous System Diseases

METHODS: Extracellular Metabolic Flux Analysis, flow cytometry-based metabolic analysis (Oxphos, mitochondrial mass, membrane potential, mitochondrial ROS production,…), high resolution confocal microscopy of mitochondria, transmission electron microscopy, microglia in vitro cultures

RESEARCH: Multi-Omics data analysis, cancer metabolism, (genetic) analyses of metabolic signaling, identification of disease biomarkers and potential therapeutic options, pipeline development for high-throughput data for clinical research and applications (e.g. Molecular Tumor Board)

METHODS: machine learning methods, development and application of standardized workflows for the analysis, integration and visualization of omics data, single-cell analysis, creating user-friendly databases

TECHNOLOGIES: High-performance computer cluster, 10x Genomics

RRSEARCH: Mitochondrial Energy Metabolism, Compartment Specific Metabolomics, Mitochondrial Diseases, Mitochondrial Protein Import and Membrane Insertion, Protein Assembly, Protein Machineries, Cell Biology, Structural Biology

METHODS: Genetic Manipulation of Metabolism, Extracellular Metabolic Flux Analysis, Untargeted Metabolomics by MS (via collaborations), Subcellular Fractionation, In vitro import of Radiolabelled Proteins into Isolated Mitochondria, Blue-native PAGE, Biochemistry

TECHNOLOGIES: Seahorse XFe96 Flux Analyzer (Agilent), Cryomill (Retsch)

RESEARCH: Acquired Metabolic Disease, Metabolic Signalling, Immunometabolism, Adaptive Immunity, T cells, Signal Transduction, Cell Biology, Inflammatory Diseases, Cardiovascular Diseases, Apolipoproteins, Autoimmunity

METHODS: Flow Cytometry, CyTOF, single cell RNA-sequencing, clinical studies, Pharmacologic and genetic manipulation of metabolism, Transcriptomics

TECHNOLOGY: Comprehensive Lab Animal Monitoring System (CLAMS)

RESEARCH: Mitochondrial Respiration & Physiology, Membrane Architecture, MICOS, F₁F_o-ATP Synthase, Respiratory Chain, Membrane Contact Sites, Mitochondrial Biogenesis & Dynamics, Mitochondrial Phospholipids, Biochemistry

METHODS: OXPHOS activity assays, Membrane Potential, Native Complex Assembly / Stability by BN-PAGE, Protein-Protein Interactions, Molecular Biology; Membrane Architecture & Dynamics by EM and Fluorescence Microscopy, Mass Spectrometry (via collaborations)

TECHNOLOGIES: OXPHOS activity assays, Native Complex Assembly / Stability by BN-PAGE, Protein-Protein Interactions

RESEARCH: Immunometabolism, Cancer Metabolism, Mitochondria, Innate Immune Sensing, Macrophages, Signal Transduction, Cell Biology, Cell Death, Inflammation, Crosstalk between cell death signalling and metabolism, Authophagy, metabolic regulation of transcription factors in AML

METHODS: Pharmacologic and Genetic Manipulation of Metabolism, Fluorescence microscopy, Extracellular Metabolic Flux Analysis, CRISPR-Cas9

RESEARCH: Medical Imaging, MRI, Method Development, X-nuclear MRI, Oxygen-17 MRI, Tumor Metabolism, Metabolic Rate of Oxygen Consumption, Warburg Effect

METHODS: Dynamic ¹⁷O-MRI, image reconstruction, modeling of oxygen uptake and metabolism, parameter analysis, radio-frequency coil construction

TECHNOLOGIES: Clinical 3T MRI Systems (Siemens PRISMA), radio-frequency laboratory, post-processing algorithms

RESEARCH: Immunometabolism, Cancer Metabolism, Metabolic Signalling, Mitochondria, Innate Immune Sensing, Macrophage Signal Transduction and Cell Biology, Cell Death, Inflammatory, Infectious, Cardiovascular and Nervous System Diseases

METHODS: Pharmacologic and Genetic Manipulation of Metabolism, Extracellular Metabolic Flux Analysis, Small Molecule Screening, Untargeted Metabolomics by MS and NMR (outsourced and via collaborations)

TECHNOLOGIES: Seahorse XFe96 Flux Analyzer (Agilent), Hypoxic Workstations i2 and H35 (Whitley), Multidrop Reagent Dispenser (Thermo)

RESEARCH: Hematopoietic Stem Cell Metabolism, Cancer Stem Cell Metabolism, Metabolic Signalling, Interplay Metabolism-Epigenetics, Dietary Habits and Stem Cell Fate, Quiescence

METHODS: Dietary, Pharmacologic and Genetic Manipulation of Metabolism, Untargeted and Targeted (low input 10,000 cells) Metabolomics by MS, Extracellular Metabolic Flux Analysis

RESEARCH: Metabolic pathway mapping, metabolic reprogramming, metabolic syndrome, tumor marker and biomarker research, crosstalk signalling and metabolism, metabolomics in personalized medicine

METHODS: Untargeted and targeted metabolome analyses via multidimensional Mass Spectrometry and NMR Analysis.

TECHNOLOGIES:  Mass Spectrometry platform in coupling with chromatography and capillary electrophoresis: Agilent QqTOF-MS, 2 Agilent QqQ-MS, Agilent GC-MS, Agilent IT-MS, Bruker Impact QqTOF-MS, Waters Synapt HR-Q(IMS)TOF-MS, 2 Thermo Orbitrap MS, Thermo GC-MS (NMR Analysis in collaboration with the Institute of Organic Chemistry)

RESEARCH: Genetic determinants of Metabolite Levels, Metabolite Transport Proteins, Metabolite handling in Kidney, Inborn Errors of Metabolism, Complex metabolic Diseases, Pharmaco-metabolomics

METHODS: Population-scale genetic screens, whole-exome sequencing, multi-Omics data integration (e.g., metabolomics and genomics), non-targeted metabolomics by MS, statistical data analysis of (non-)targeted MS and NMR metabolomics, pathway analysis

RESEARCH: Cancer Metabolism, Autophagy, Mitochondria, Pancreatic Cancer, Targeted Therapy, Tumor Microenvironment, Immunooncology

METHODS: Indirect Interference with mutant KRAS driven tumor cell intrinsic metabolic rewiring, Pharmacologic and Genetic Manipulation, Small Molecule Evaluation, Seahorse, Targeted Metabolomics and Metabolic Flux Analysis by MS  (via collaboration)

RESEARCH: Metabolism, metabolism signaling, Natural products, antibiotics, biosynthesis of natural products, regulation, cyclic di-GMP, cofactor dependent enzymes, transport of natural products, oxygenases, glycoyltrasnferases, microorganisms in insects

METHODS: Genetic Manipulation of Metabolism, Small Molecule Structure elucidation, Characterization of Natural Products by MS and NMR, Protein structure elucidation, Cryo-EM

TECHNOLOGIES: Triple-Stage-Quadrupol-Massenspektrometer 40-500 + UnliMate 3000 Binäres HPLC-System + DAD (Thermo)